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. Author manuscript; available in PMC: 2016 Jun 1.
Published in final edited form as: FEBS J. 2015 Apr 16;282(12):2379–2393. doi: 10.1111/febs.13286

Figure 4.

Figure 4

Figure 4

A. Representative Urea-gel picture of the time course of phosphorylation of WT and D94A RLCs. Note a charge based separation of the phosphorylated (P-RLC) and non-phosphorylated RLC bands. B. Time course of MLCK-dependent phosphorylation of WT and D94A RLCs (0 to 8 minutes). Data points were fitted to Eq. 3 to generate phosphorylation kinetics rates (k). Note that both WT and D94A were phosphorylated with the rate of 1.1 min−1 (n=3 independent experiments) and reached the same maximal level of RLC phosphorylation.