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Polymerase chain reaction primers for amplification of virulence factors
| Size of PCR product (bp) | Primer sequence | Primer designation |
|---|---|---|
| 567 bp (m1) 642 bp (m2) |
vacAmF: 5-CAATCTGTCCAATCAAGCGAG-3 vacAmR: 5-GCGTCTAAATAATTCCAAGG-3 |
vacA m1/m2 |
| 259 bp (s1) 286 bp (s2) |
VA1-F: 5-ATGGAAATACAACAAACACAC-3 VA1-R: 5-CTGCTTGAATGCGCCAAAC-3 |
vacA s1/s2 |
| 232 bp | cag1: 5-ATGACTAACGAAACTATTGATC-3 cag2 5-CAGGATTTTTGATCGCTTTATT-3 |
cagA |
