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. Author manuscript; available in PMC: 2016 Jun 16.
Published in final edited form as: Cell Rep. 2015 Jun 4;11(10):1651–1666. doi: 10.1016/j.celrep.2015.05.013

Figure 2. FXR2P Deficiency Leads to Impaired Neural Network Integration of Newborn Neurons.

Figure 2

(A) Schematic illustration of pseudo-typed rabies virus-mediated monosynaptic retrograde tracing to map the presynaptic inputs to newborn neurons in the DG. RV-Syn-GTRgp, retrovirus-expressing GFP, TVA, and rabies virus GapPol proteins, driven by a neuronal synapsin promoter. Retrovirus infects only dividing cells, including neural progenitors (NPCs), in the adult DG. EnvA-mCherry, EnvA pseudo-typed rabies virus expressing mCherry can only infect new neurons expressing TVA receptor for EnvA coat protein.

(B) Schematic illustration of timeline of the rabies virus-mediated monosynaptic retrograde tracing experiment.

(C) Sample confocal images showing newborn cells (both green and red) in the DG, and traced cells (red only) in DG, hilus regions, CA regions, and cortex. Scale bar, 200 μm.

(D) High-magnification confocal images of the newborn cells (green and red) and traced cells (red alone) in the DG. Scale bar, 50 μm.

(E, F) The ratio of “Traced Cells (mCh+)” to “Starter Cells” (GFP+mCh+) in total brain (E, n = 5 mice/condition, P = 0.0052; WT: 24 GFP+mCh+ cells/mouse; 85 mCh+ cells/mouse; KO: 27 GFP+mCh+ cells/mouse; 28 mCh+ cells/mouse) or in specific brain regions (F, GC, WT, 24 mCh+ cells/mouse, KO 8 mCh+ cells/mouse, p = 0.0056; cortex cells, CTX, WT, 43 mCh+ cells/mouse, KO, 14 mCh+ cells/mouse, p = 0.005; hilar cells, HC, WT, 29 mCh+ cells/mouse, KO, mCh+ cells/mouse, P = 0.005; and CA cells, WT, 5 mCh+ cells/mouse, KO, 2 mCh+ cells/mouse, p = 0.006) of Fxr2 KO mice compared to WT mice. **, p <0.01.