Fig 6.

ASXL2−/− mice are lipodystrophic. A) Weight of BAT, eWAT and scWAT in WT and ASLX2−/− mice. B) Fat/Lean ratio of WT and ASXL−/− mice. C) WT and ASXL2−/− mice were injected with insulin (5U/Kg) or PBS. 10 min later phosphorylation of Akt in epididymal fat was determined by immunoblot. D) WT and ASXL2−/− adipocyte size measured by osmium tetroxide staining. E) Adipocyte number in WT and ASXL2−/− epididymal fat pad. F) mRNA of adipogenic genes in ASXL2−/− and WT eWAT was measured by qPCR. G) Marrow stromal cells of WT and ASXL2−/− mice were cultured in adipogenic conditions for 14 days. The cells were stained with oil red O (red reaction product) to identify lipid. H) eWAT was treated with DMSO or isoproterenol for 1 h. Media glycerol content was determined. I) mRNA of lipid storage genes in WT and ASXL2−/− eWAT was measured by qPCR. J) Serum triglycerides and cholesterol of chow-fed WT and ASXL2−/− mice. Scale Bar: 50 μm.