Figure 5. Relationship between CACNA1C and CREB1 or C-MYC expression in METH-treated and untreated cells after 48 hr drug treatment.
A. CREB1 expression is significantly higher (p=0.03 by unpaired t-test) in METH-treated cells (n=13) compared to untreated cells (n=13) (left panel). CREB1 expression levels are positively correlated to CACNA1C expression levels in METH-treated cells but inversely correlated in untreated cells (right panel: ANCOVA, p=0.04).
B. Expression levels of MYC gene in METH-treated cells (n=14) is not significantly different (p=0.2 by unpaired t-test) than those found in untreated cells (n=14) (left panel). The right panel shows the scatter plot when data was stratified according to drug treatment; CACNA1C expression is significantly correlated with the expression levels of C-MYC genes (overall r=0.7, p=0.0002). However, the slopes of METH and untreated cells were not different from each other (ANCOVA p=0.5).