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. Author manuscript; available in PMC: 2015 Jun 19.
Published in final edited form as: Circulation. 2012 Dec 24;127(4):476–485. doi: 10.1161/CIRCULATIONAHA.112.132126

Figure 3.

Figure 3

Platelet function of STAT3Δ/Δ mice: Platelets from pSTAT3Δ/Δ and STAT3F/F littermates were induced to aggregate by 0.5, 0.75, or 5 μg/ml of collagen (A-C) and data from 32 mice/group were quantified (D, *p < 0.01). CD62p expression was measured after platelets were stimulated with 0.5, 0.75 or 5 μg/ml of collagen. The comparison was made between WT and STAT3 KO platelets at each collagen level with a two-way ANOVA (E, n = 12, *p < 0.001). No interaction between litter and genotype was found at any of these collagen doses. Calcium influx was detected in platelets stimulated with 0.75 μg/ml of collagen (F, *p < 0.003). Blood was perfused over immobilized collagen for 10 min at a flow rate of 1 ml/min to measure thrombus formation (G, representative images, bar = 50 μm), which was quantified by measuring surface areas covered by platelet thrombi (H, n = 10, *p < 0.001).