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. Author manuscript; available in PMC: 2015 Jun 19.
Published in final edited form as: Circulation. 2012 Dec 24;127(4):476–485. doi: 10.1161/CIRCULATIONAHA.112.132126

Figure 6.

Figure 6

Effect of IL-6 on platelet and HEK293 cells. STAT3 phosphorylation was measured in platelets stimulated with IL-6/sIL-6R or IL-6 in the absence (A) and presence (B) of increasing concentrations of collagen. HEK293 cells transiently expressing human Syk and PLCγ2 were stimulated with 10 ng/ml of IL-6 for 1 hr at 37°C and then lysed. Cell lysates were probed for total and phosphorylated STAT3 (C). These cells were also stimulated with IL-6 in the presence of STA21 and probed for STAT3 phosphorylation (D). Resting and IL-6 stimulated HEK293 cells were lysed and incubated with a STAT3 antibody followed by immunoprecipitation by protein A coupled beads (E). Immunoprecipitated proteins were probed for Syk, PLCγ2, and STAT3. Non-immune isotype IgG was used as negative control. The figure represent of 3–4 separate experiments.