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. 2015 Jun 18;10(6):e0129810. doi: 10.1371/journal.pone.0129810

Fig 1. The multi-target artificial positive control (APC).

Fig 1

Top, APC made by a custom synthesized DNA insert containing tandems of forward, reverse and probe complement priming sequences ligated into the multiple cloning site (MCS) in the vector pUC57. Each amplified PCR product using APC has a unique identifiable sequence. Bottom, the product sizes of the targets differ from the product size and sequences amplified using genomic DNA. A/B, where A is amplicon size generated using genomic DNA of target species and B is amplicon size generated using APC (shown in picture). Different colors at track 1 are the indications of reporter dyes wavelengths (excitation and emission spectra) detected by different channels of real-time qPCR for particular species of Liposcelis as shown in Table 3. Primers and probes sequences indicated by track 1 were used in this study. Primers and probes sequences indicated by track 2 are from fungi, viruses and insect and were not used in this study. Lists of primer and probe sequences are given in Tables 2 and 3, respectively.