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. 2015 Jun 18;10(6):e0129810. doi: 10.1371/journal.pone.0129810

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© 2015 Arif et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Fig 2 — Assay was performed with insect genomic DNA and primer sets ObsCo13F/13R (438 bp), PeaCo15F/14R (351 bp), BosCO7F/7R (191 bp), BruCo5F/5R (140 bp), and DecCo11F/11R (87 bp) for L. obscura, L. pearmani, L. bostrychophila, L. brunnea and L. decolor, respectively. Lane 1: PCR reaction with all five species genomic DNA. Lanes 2–6: single species genomic DNA viz. L. obscura, L. pearmani, L. bostrychophila, L. brunnea and L. decolor, respectively. Lanes M and W are 1 kb ladder and non-template control (water), respectively.