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. 2015 Jun 18;10(6):e0129810. doi: 10.1371/journal.pone.0129810

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© 2015 Arif et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Fig 4 — Assay was performed with 10-fold serially diluted multi target artificial positive control (APC) from 1 ng to 1 fg using primer and probe sets (A) ObsCo12F/12R/12P (72 bp), (B) BruCo5F/5R/5P (140 bp), (C) BosCo8F/8R/8P (96 bp), (D) PeaCo14F/14R/14P (67 bp), and (E) DecCo11F/11R/11P (99 bp). Different channels viz. orange, yellow, red, crimson and green corresponding to the different reporter dye (excitation/emission spectra in nm) viz. 6-ROXN (575/602), HEX (535/554), Cy5 (647/667) and Quasar705 (690/705) and 6-FAM (495/520), respectively.