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. 2015 Jun 18;10(6):e0129810. doi: 10.1371/journal.pone.0129810

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© 2015 Arif et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Fig 5 — Standard graphs were generated using 10-fold serially diluted multi target artificial positive control (APC) from 1 ng to 1 fg using the primer and probe sets BruCo5F/5R/5P (140 bp), BosCo8F/8R/8P (96 bp), DecCo11F/11R/11P (99 bp), ObsCo12F/12R/12P (72 bp) and PeaCo14F/14R/14R (67 bp). Different channels viz. orange, yellow, red, crimson and green corresponding to different reporter dye (excitation/emission spectra in nm) viz. 6-ROXN (575/602), HEX (535/554), Cy5 (647/667) and Quasar705 (690/705) and 6-FAM (495/520).