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. 2015 Jun 18;10(6):e0130322. doi: 10.1371/journal.pone.0130322

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© 2015 Chen et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Fig 4 — AKTi (A) or MG132 (B) was added 1 hour before TSH stimulation of healthy cultured fibrocytes. RNA was isolated after 6 hours of induction. AKTi treatment leads to significant reduction in TSH-induced TNFα mRNA expression (from 23-fold to 1.3-fold expression, p <0.0001) (A). Similarly, MG132 treatment inhibits TSH-induced TNFα mRNA expression (from 93-fold to 16-fold expression; p< 0.001) (B).