Skip to main content
. 2015 Jun 18;10(6):e0129648. doi: 10.1371/journal.pone.0129648

Fig 1. Protein and activity levels of p210BCR-ABL and Separase expression in cell lines under investigation.

Fig 1

Protein levels of p210BCR-ABL (A), pCrkL (B) and Separase (C) based on densitometric evaluation of immunostained Western blots were normalized to Actin as loading control. Abl-related TK activity (BCR-ABL + c-ABL) was measured as pCrkL/Actin (B). Analyses were performed on protein lysates derived from p210BCR-ABL-positive (KCL-22, BV-173, LAMA-84, K562) and-negative non-malignant cells (NHDF, UROtsa). KCL-22 and BV-173 carry the bcr-abl breakpoint variant b2a2, whereas LAMA-84 and K562 display the b3a2 fusion gene variant. All values refer to that of K562 (= 100%).