Table 3. Origin and characteristics of human cell line models under investigation.
| Cell line | Cell type | Origin | Doubling time [h] 1 | BCR-ABL-copy no/ type | centrosomal status [%] 2 | Cytogenetics | Used as model for |
|---|---|---|---|---|---|---|---|
| NHDF | normal human dermal fibroblasts, primary | juvenile foreskin, from healthy donor | ~ 72 | none | normal, < 5% | 46,XY | normal cells |
| UROtsa | human urothelial | normal urothelial cells immortalized with SV40 | ~ 63 | none | normal, < 3% | 46,XX | normal cells |
| KCL-22 | human CML (BC) | pleural effusion of patient with CML BC, myeloblastic | ~24 | 2 / (b2a2) | aberrant, 78% | human hyperdiploid karyotype with 3.3% polyploidy– 51(47–51)<2n>X,-X, +1, +6, +8, +8, +14, +22, del(1)(p22), t(9;22)(q34;q11), add(17)(p12-13), i(21q), der(22)t(9;22)(q34;q11)–carries Phx2 –matches published karyotype (DSMZ) | CML, b2a2 |
| BV-173 | human CML (BC) | PB of a patient with CML BC, lymphoblastic | ~48 | 1 / (b2a2) | aberrant, 10% | human hyperdiploid karyotype– 47(46–48)<2n>X/XY, -9, +22, +mar, add(1)(q42), add(8)(p23), t(9;22)(q34;q11), der(22)t(9;22)(q34;q11), der(?)t(9;?)(?p11;?) (DSMZ) | CML, b2a2 |
| LAMA-84 | human CML (BC) | PB of a patient with CML BC, myeloblastic | ~ 50 | 4 / (b3a2) | aberrant, 43% | 73/74(69–77)<3n>XX,-X,+1,-2,+5,+6,+8,+13,-14,+17,+17,-18,+22,+mar,del(7)(p15),der(9)t(9 ;22)(q34 ;q11)x2,i(11q), add (13)(q33),del(17)(p12),der(22)t(9 ;22)(q34 ;q11)x4 (DSMZ) | CML, b3a2 |
| K562 | human CML (BC) | pleural effusion of a patient with CML BC, lymphoblastic | ~ 36 | 11 / (b3a2) | aberrant, 34% | 61–68<3n>XX,-X,-3,+7,-13,-18,+3mar,del(9)(p11/13), der(14)t(14;?)(p11;?),der(17)t(17;?)(p11/13;?),der(?18) t(15;?18)(q21;?q12),del(X)(p22) (DSMZ) | CML, b3a2 |
Abbreviations: PB, peripheral blood; CML, chronic myeloid leukemia; CP, chronic phase; BC, blast crisis; no, number; DSMZ, German Collection of Microorganisms and Cell Cultures, Braunschweig, Germany
1 doubling time of subconfluent cells
2 Analysis of 100 cells by immunofluorescence microscopy after centrosome (pericentrin) staining