Fig 4. Deletion of SFP1 enhances C. albicans biofilm formation.

(A) Biofilms were formed on the surface of a 24-well polystyrene microplate in SC medium at 37°C with 5% CO₂ for 24 h. (B) Biofilm formation was assessed using the XTT reduction assay. The results are presented as the mean ± SD from three independent experiments. *p < 0.05 for sfp1Δ/sfp1Δ vs. WT cells. (C) Biofilm structure was examined using scanning electron microscopy with a 1,000× magnification. Biofilm formation was carried out as described above, except the cells were grown on polystyrene coverslips for 24 h.