Table 1. Probability of identity (PI) and measures of genetic diversity at 12 microsatellite loci in our capercaillie population sorted by polymorphic information content (PIC).
Locus | N | A | R | Ho | He | PIC | PI | PIsib | HW | FNull |
---|---|---|---|---|---|---|---|---|---|---|
sTuD7-FAM | 119 | 2 | 2 | 0.639 | 0.489 | 0.369 | 0.382 | 0.602 | 0.001* | -0.135 |
sTuD3-NED | 119 | 5 | 5 | 0.496 | 0.517 | 0.444 | 0.306 | 0.569 | 0.076 | 0.017 |
sTuT1-VIC | 118 | 4 | 3.992 | 0.364 | 0.511 | 0.456 | 0.294 | 0.569 | 0.000* | 0.154 |
sTuT3-FAM | 120 | 5 | 5 | 0.492 | 0.527 | 0.469 | 0.282 | 0.558 | 0.555 | 0.036 |
sTuD6-NED | 119 | 12 | 11.966 | 0.571 | 0.528 | 0.509 | 0.241 | 0.548 | 0.728 | -0.061 |
BG15-FAM | 117 | 5 | 5 | 0.607 | 0.597 | 0.527 | 0.232 | 0.511 | 0.069 | -0.007 |
sTuT2-VIC | 120 | 5 | 4.999 | 0.533 | 0.586 | 0.538 | 0.219 | 0.513 | 0.008 | 0.043 |
sTuD1-NED | 120 | 6 | 5.975 | 0.708 | 0.722 | 0.669 | 0.129 | 0.423 | 0.099 | 0.011 |
sTuD4-NED | 118 | 8 | 7.991 | 0.678 | 0.741 | 0.697 | 0.11 | 0.409 | 0.196 | 0.044 |
sTuT4-PET | 120 | 5 | 5 | 0.683 | 0.769 | 0.727 | 0.093 | 0.391 | 0.136 | 0.055 |
BG18-FAM | 120 | 7 | 6.95 | 0.817 | 0.781 | 0.743 | 0.085 | 0.382 | 0.068 | -0.025 |
sTuD5-PET | 120 | 10 | 9.974 | 0.833 | 0.859 | 0.838 | 0.038 | 0.332 | 0.539 | 0.012 |
Across loci | 6.167 (0.787) | 6.154 (0.783) | 0.618 (0.04) | 0.636 (0.038) | 0.582 (0.042) | 5.0582E-10 | 1.3403E-04 |
Across loci = means (and SE) over loci for A, R, Ho and He, and combined probability for PI and PIsib over all loci. HW = p-value of exact test [32], with asterisks indicating significant deviation from HW expectations at a Bonferroni-corrected α-level of 0.0042 (p-value of 0.05 divided by 12). N = number of samples, A = number of alleles, R = allelic richness (based on a minimum sample size of 117 individuals), Ho = observed heterozygosity, He = expected heterozygosity, PIC = polymorphic information content, a measure of informativeness related to expected heterozygosity and calculated from allele frequencies [31], PI = probability of identity, PIsib = probability of identity considering the possible presence of siblings (see [30]), FNull = Frequency of null alleles per locus; negative values indicate excess of observed heterozygote genotypes.