Fig. 3.

Recovery of viable VP6-truncated IBAV from BHK21A11 cells. (A) The secondary structure of IBAV VP6 protein was predicted using Jpred 4 system (http://www.compbio.dundee.ac.uk/jpred/). The aa sequence of IBAV VP6 and the final prediction (Jnet) were shown on the top and second lane, respectively. Numbers indicates aa position. The hidden markov model based prediction (jhmm) and the position specific score matrix based prediction (jpssm) were also shown. The positions of ß-strands and α-helix were indicated as ‘E’ and ‘H’, respectively. (B) Genomic dsRNA was purified from infected cells and analyzed by nondenaturing-PAGE. White arrows indicate the position of the corresponding S9 in each VP6-truncated virus. (C) Virus growth kinetics of VP6-truncated viruses IBAVd1 and IBAVd2. Total virus titer was determined by plaque assay at 0, 12, 24, 48, and 72 h post-infection and plotted as PFU/ml in logarithmic scale (Mean ± SD). As a control, cells were infected with WT IBAV-2.