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. 2015 May 27;5:445–453. doi: 10.1016/j.fob.2015.05.006

Fig. 4.

Fig. 4

Characterization of VP6-truncated viruses in normal BHK21A11 cells. (A) Each 100 μl of VP6-truncated IBAV plaque-purified twice from BHK21A11 cells were once amplified in BHK21A11 cells (P2), followed by amplification 9 times (P3–P11) in BSR cells. Pattern of genomic dsRNA purified from cells infected with IBAVd1 and IBAVd2 (P2, P6, P11) was analyzed by nondenaturing-PAGE. Position of the corresponding S9 in each VP6-truncated virus is indicated with white arrows. (B) Core particles purified from BSR cells infected with each of P11 IBAV were resolved by SDS–PAGE. Position of the corresponding VP6 in each VP6-truncated virus is indicated with black arrows. (C) Electron microscopy of core particles of each VP6-truncated virus. Bar: 100 nm.