Fig. 5.

Recovery of viable VP6-tagged IBAV from BHK21A11 cells. (A) Schematic representation of the changes introduced in IBAV VP6. On the left, the name of mutation is indicated. Numbers indicate amino acid positions in VP6 where deletions were introduced. Several amino acid sequences for GAGAG, Flag, HA, and TC were inserted into a truncated region of the first loop. (B) Genomic dsRNA was purified from infected cells and analyzed by nondenaturing-PAGE. White arrows indicate the position of the corresponding S9 in each VP6-tagged virus. (C) Virus growth kinetics of VP6-tagged viruses IBAVd1Flag, HA and TC. Total virus titer was determined by plaque assay at 0, 12, 24, 48, and 72 h post-infection and plotted as PFU/ml in logarithmic scale (Mean ± SD). As a control, cells were infected with WT IBAV-2.