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. 2015 May 27;5:445–453. doi: 10.1016/j.fob.2015.05.006

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© 2015 The Authors

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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Fig. 6 — Characterization of VP6-truncated viruses in normal BHK21A11 cells. (A) Either Flag or HA-tagged VP6 were detected in BSR cells infected with VP6-tagged IBAV by immunoblot analysis. As a control, IBAVd1 or IBAVd1TC were infected to the cells. Arrowheads indicate the detected tagged proteins. (B) TC-tagged VP6 was detected in the IBAVd1TC-infected BSR cells using FlAsH-EDT₂ labeling reagent. Note that some TC-VP6 proteins were detected as puncta (white arrowheads). (C) After 10 times passage of each VP6-tagged IBAV, pattern of genomic dsRNA purified from cells infected with IBAVd1Flag, HA and TC (P2 and P10) was analyzed by nondenaturing-PAGE. Position of the corresponding S9 in each VP6-tagged virus is indicated with white arrows.