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Processing of the 5’ strand (upper panels) and 3’ strand (lower panels) of AgoshGag5 and AgoshPol1 variants was analyzed by Northern blot analysis. The asterisks indicate the regular Dicer processed small RNAs of 21 nt and the Ago2 products of ~33 nt. The RNA structure cartoon indicates which side of the hairpin was probed for AgoshRNAs and the control shRNAs.
