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. 2015 Jun 18;10(6):e0128618. doi: 10.1371/journal.pone.0128618

Fig 7. Knockdown activity of the AgoshRNA 5’ guide on HIV-1 production.

Fig 7

The knockdown activity of the 5’ strands of the AgoshGag5 (upper graph) and AgoshPol1 variants (lower graph) were tested by co-transfection of 2.5, 10 or 40 ng of AgoshRNA construct together with 250 ng of the full-length HIV-1 molecular clone pLAI and 1 ng of Renilla plasmid. The CA-p24 levels in the supernatant were determined as a measure for HIV-1 production. HIV-1 production in the vector transfected cell culture was set at 100%. We performed three independent transfections, each in duplicate, and standard deviations were calculated.