Table 1. Stability and potency of the hairpins.
name | Complete hairpin | Top half | Bottom half | Luciferase knockdown | |
---|---|---|---|---|---|
ΔG (kcal/mole) | sense | antisense | |||
AgoshGag5 | -27.7 | -7.2 | -12.8 | +++ | - |
GU-AgoshGag5 | -19.8 | -6.5 | -5.6 | - | - |
AgoshPol1 | -33.3 | -13.4 | -12.8 | + | - |
GU-AgoshPol1 | -24.7 | -9.0 | -8.6 | - | - |
AgoshPol47 | -32.2 | -14.1 | -10.4 | - | - |
GU-AgoshPol47 | -30.2 | -12.8 | -9.7 | - | - |
AgoshRT5 | -37.0 | -14.1 | -16.3 | + | - |
GU-AgoshRT5 | -29.4 | -11.2 | -12.2 | + | - |
Inhibition;-, no; +, 20–40%; ++, 40–70%; +++, 70–100%.