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Six colorectal cancer FFPE blocks labeled C17–C22 were deparaffinized, homogenized and protein are extracted according to the protocol described above, a total of 25 μl of each sample is resolved using Western blotting analysis which demonstrated the expression of the protein Actin compared to control actin isolated from MDA 231 cell lines. Molecular weight (in kDa) is shown on the left side of the figure.
