Fig. 1.

Effect of AECHL-1 on survival and proliferation of HUVECs. a HUVECs treated with varying concentrations of AECHL-1 for 48 h, and cell viability was determined by MTT assay. b HUVECS treated with varying concentrations of AECHL-1 for 24 h and subjected to flow cytometry using Annexin V-FITC as a marker of apoptosis. c HUVECs treated with varying concentrations of AECHL-1 for 48 h, and cell proliferation was determined by tritiated thymidine assay. Columns, mean from three independent experiments performed in triplicate; bars, SE. *P < 0.05, ***P < 0.001 versus control alone