Fig. 6.

Effect of AECHL-1 on VEGFR2-mediated MAPK signaling. a Quantification of VEGFR2 phosphorylation by ELISA. Fold change indicates the ratio of phosphorylated to nonphosphorylated VEGF. Cells were pretreated with AECHL-1 for 4 h and stimulated with VEGF for 5 min. b Cells were pretreated with AECHL-1 for 4 h and stimulated with VEGF for 25 min. Cells were harvested, and Western blotting was performed for detection of pPLC-γ1, pMEK1/2 and pERK1/2. Membrane was stripped and reprobed with Total MEK1/2, ERK1/2 and GAPDH to indicate equal loading. Columns, mean from three different experiments performed in triplicate; bars, SE