Fig. 7.

Effect of AECHL-1 on microfilament dynamics and motility-associated proteins. HUVECs were grown on coverslips till confluency and scratched with a pipette tip. Cells were pretreated with AECHL-1 for 4 h and then stimulated with VEGF and incubated for 9 h. Coverslips were then fixed and processed for immunostaining. Immunofluorescence analysis of HUVECs stained with phalloidin conjugated with Alexa Fluor 488 and a anti-IQGAP1 antibody b anti-WAVE-2 antibody. Membrane ruffles indicate active lamellipodial edge (white arrow). Images were taken by a confocal microscope at ×60 magnification. c Also at the indicated time (9 h), cells were harvested and subjected to Western blotting for detection of WAVE-2 and pRac/Cdc42. Membrane was stripped and reprobed with total Rac/Cdc42 and GAPDH to indicate equal loading