Fig. 5.

Human insulin is detectable in grafts of long-term cryopreserved human islets after 10 weeks. (a, b) Anti-insulin HRP/DAB staining of renal sub-capsular grafts of long-term cryopreserved human islets. (c) Insulin (red) and glucagon (green) immunofluorescence and DAPI staining (blue) in a section of a graft of cryopreserved islets removed after 10 weeks. (d–h) Insulin immunostaining (red) and DAPI staining (blue) of grafts of freshly isolated islets from three separate donors (d) and long-term cryopreserved islets from four separate donors (e–h). The cryopreserved islets shown were from donors that reduced the blood glucose level in recipient mice to 12.4 (e), 20.4 (f), 26.3 (g) and 23.4 (h) mmol/l. Scale bar, 100 μm (a) or 200 μm (b–e)