Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2015 Jun 19;6:118. doi: 10.3389/fphar.2015.00118

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2015 O’Leary and Chahine.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

Methanethiosulfonate reagent inhibition is voltage-dependent inhibition. The MTSET inhibition of the V1763C (A) and Y1767C (B) mutants was examined while varying the holding potential between –140 and –100 mV. Standard test pulses to –10 mV for 20 ms were applied at 5 s intervals to assess current amplitude. The test currents were normalized to the current measured immediately after break-in. Plotted are the normalized currents for the V1763C and Y1767C mutants in the absence and presence of 1 mM internal MTSET. Holding at a more depolarized voltage (–100 mV) accelerated the onset of the MTSET inhibition by comparison to the –140 mV holding potential. The data are the means and SEM of the V1763C Control (n = 9), V1763C MTSET (n = 4), Y1767C control (n = 9), and Y1767C MTSET (n = 14).