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. 2015 Jun 19;6:118. doi: 10.3389/fphar.2015.00118

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Copyright © 2015 O’Leary and Chahine.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The F1760C mutant alters closed-state inactivation. The onset of closed-state inactivation was measured by applying variable length prepulses (0–150 ms) to voltages between –110 and –70 mV. The cells were then returned to –140 mV for 1 ms before assessing availability with a standard test pulse to –10 mV for 20 ms. The peak currents were normalized and plotted versus the prepulse pulse duration. (A,B) Onset of closed-state inactivation of the wild-type and F1760C channels. The smooth curves are fits to single exponential functions. (C) Time constants of the wild-type and F1760C mutant obtained from panels (A,B) plotted versus the prepulse potential. The smooth curve is a fit to a two state model yielding the inactivation (β) and recovery (α) rate constants (See Materials and Methods). (D) Plot of β and α as a function of voltage.