Fig. 1.

(A) Flow cytometric analysis of a 21-day-old mouse testicular cell suspension stained with VDG. A dot plot depicting forward scatter (FSC-H) vs. VDG fluorescence intensity and its corresponding histogram are shown. Peaks corresponding to cell populations differing in their DNA content (C, 2C and 4C) are indicated. Sorting gates chosen within the C and 4C populations are shown as well (R1 and R2, respectively). (B) Analysis of sorted cells from the indicated gates (C upper region [R1], and 4C right upper region [R2]). Sorted cells were analyzed under laser confocal (VDG green fluorescence) and differential interference contrast (DIC) microscopy. Note the homogeneity and integrity of the classified material. Bars correspond to 20 μm.