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. 2015 Jun 18;10(6):e0129994. doi: 10.1371/journal.pone.0129994

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© 2015 Scandolera et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Fig 4 — Young, intermediate and aged fibroblasts were represented by passage 3 (white), 5 (grey) and 8 (black bars). (A) Analysis of Neu-1 and PPCA protein levels during aging. Cells were stimulated or not for 24 hours with 50 μg/mL of EDP before protein extraction. Neu-1 and PPCA were then analyzed by Western blotting using anti-Neu-1 and anti-PPCA antibodies. Densitometric analysis was performed using the Quantity One software. The results were expressed as the ratio Neu-1/β-actin and PPCA/β-actin and normalized to the control expressed in percent. Dotted lines (100%) represent the values of the unstimulated controls. (B) Analysis of Neu-1 and PPCA localization during aging. Neu-1 and PPCA cell localizations were analyzed by confocal microscopy. Bar, 10 μm.