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. 2015 Jun 18;10(6):e0129994. doi: 10.1371/journal.pone.0129994

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© 2015 Scandolera et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Fig 6 — Young, intermediate and aged fibroblasts were represented by passage 3 (white), 5 (gray) and 8 (black), respectively. (A) Basal LacCer production increases with age. LacCer production was detected by flow cytometry using a specific FITC-coupled anti-LacCer antibody (anti-CD17). DANA was used to study the impact of sialidase inhibition on basal production of LacCer during in vitro aging. (B) EDP-mediated LacCer production is altered with age. Cells were stimulated or not with 50 μg/mL of EDP for 30 minutes in the presence or not of DANA. LacCer production was then analyzed by flow cytometry as described above. Histograms represent the normalized results relative to the control expressed in percent. Each data is representative of three independent experiments. *, p<0.05, ***, p<0.01. Dotted lines represent the values of the unstimulated controls.