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The four RIF heteroresistant isolates detected by DNA sequencing showed dual peaks of wild type (WT) and mutant bases at certain codons (circled). The phenotypic DST for RIF: RIFr (resistant to RIF) and RIFs (susceptible to RIF). (a to c) WT and secondary mutation bands at codons 526 (TGC), 531 (TTG), and 526 (TAC), respectively. (d) Main peak with mutant base (CCG) and WT (CTG) at codon 511.
