TABLE 2.
MLST of Hib isolates and reference isolates used for creation of Hib/non-Hib classification methods
| Isolate | Capsular type | MLST results |
Location | Yr | Site of isolation | |||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| adk | atpG | frdB | fucK | mdh | pgi | recA | ST | |||||
| MLST of Hib isolatesa | ||||||||||||
| G4 | b | 10 | 14 | 4 | 5 | 4 | 7 | 8 | 6 | Gothenburg | 2002 | Blood |
| G6b | b | 31 | 14 | 4 | 5 | 4 | 7 | 8 | 95 | Gothenburg | 2004 | Blood |
| G26 | b | 10 | 14 | 4 | 5 | 4 | 7 | 8 | 6 | Gothenburg | 2009 | Blood |
| G32 | b | 10 | 14 | 4 | 5 | 4 | 7 | 8 | 6 | Gothenburg | 2008 | CSF |
| KR169b | b | 10 | 14 | 4 | 5 | 4 | 7 | 8 | 6 | Stockholm | 1998 | Blood |
| KR181 | b | 31 | 14 | 4 | 5 | 4 | 7 | 8 | 6 | Stockholm | 1998 | CSF |
| KR191b | b | 10 | 14 | 5 | 7 | 57 | 7 | 8 | 120 | Stockholm | 2004 | Blood |
| KR194b | b | 6 | 30 | 23 | 9 | 33 | 29 | 7 | New STc | Stockholm | 2004 | Blood |
| KR209 | b | 10 | 14 | 4 | 5 | 4 | 7 | 8 | 6 | Stockholm | 2009 | Blood |
| KR211 | b | 10 | 14 | 4 | 5 | 4 | New | 8 | New STd | Stockholm | 2009 | Blood |
| L2 | b | 10 | New | 4 | 5 | 4 | 7 | 8 | New STe | Lund | 2004 | Blood |
| L35 | b | 10 | 14 | 4 | 5 | 4 | 7 | 8 | 6 | Lund | 2005 | Blood |
| L40 | b | 10 | 14 | 4 | 5 | 4 | 7 | 8 | 6 | Lund | 2004 | Blood |
| L58 | b | 10 | 14 | 4 | 5 | 4 | 7 | 8 | 6 | Lund | 2007 | Blood |
| L62b | b | 10 | 14 | 4 | 5 | 4 | 7 | 8 | 6 | Lund | 2007 | Blood |
| L63 | b | 10 | 14 | 4 | 5 | 4 | 7 | 8 | 6 | Lund | 2008 | Blood |
| M16b | b | 10 | 14 | 4 | 1 | 4 | 7 | 8 | 206 | Malmö | 2007 | Blood |
| M17b | b | 10 | 14 | 5 | 7 | 4 | New | 8 | New STd | Malmö | 2007 | Blood |
| M41 | b | 10 | 14 | 4 | 5 | 4 | 7 | 8 | 6 | Malmö | 2004 | Blood |
| Non-Hib reference isolates | ||||||||||||
| KR152f | a | 20 | 12 | 25 | 7 | 20 | 23 | 19 | 30 | Unknown | Unknown | Unknown |
| KR153f | c | 7 | 11 | 6 | 8 | 6 | 16 | 9 | 9 | Unknown | Unknown | Unknown |
| KR154f | d | 5 | 15 | 7 | 9 | 7 | 5 | 11 | 47 | Unknown | Unknown | Unknown |
| L13 | e | 18 | 6 | 3 | 7 | 10 | 28 | 12 | 18 | Lund | 2006 | Blood |
| M1 | f | 22 | 19 | 11 | 11 | 22 | 19 | 15 | 124 | Malmö | 2009 | Blood |
| G23 | NTg | 5 | 1 | 1 | 1 | 1 | 2 | 5 | 14 | Gothenburg | 2008 | Blood |
| G63 | NT | 14 | 7 | 13 | 7 | 17 | 13 | 17 | 57 | Gothenburg | 2010 | Blood |
| KR206 | NT | 11 | 2 | 15 | 8 | 28 | 26 | 3 | 34 | Stockholm | 2008 | Blood |
| L1 | NT | 1 | 1 | New | 14 | 9 | 14 | 3 | New STh | Lund | 2004 | Blood |
| M15 | NT | 1 | 1 | 1 | 1 | 7 | 2 | 5 | New STi | Malmö | 2008 | Blood |
MLST was performed on 19 Hib isolates. The selection of Hib reference isolates was based on MLST and spectral characteristics. Hib not selected as reference isolates were used as test isolates for Hib/non-Hib classification methods. Non-Hib reference isolates and their characteristics are also presented.
Selected as Hib reference isolate.
Most similar to ST93 among previously known STs.
Most similar to ST53 among previously known STs.
Most similar to ST6 among previously known STs.
Unfortunately, for the Hia, Hic, and Hid reference isolates, the location, year, and tissue from which they were isolated are unknown.
NT, nontypeable.
Most similar to ST103 among previously known STs.
Most similar to ST3 among previously known STs.