TABLE 3.
Background variation in the in vitro-transcribed RNA samples
No. of in vitro-transcribed RNA molecules per reaction | Avg % nt variation ± 95% CI | Maximum nt variation (%)a |
---|---|---|
108 (500 pg) | 0.28 ± 0.0049 | 4.5 |
107 (50 pg) | 0.34 ± 0.0078 | 5.3 |
106 (5 pg) | 0.28 ± 0.0039 | 2.8 |
105 (500 fg) | 0.16 ± 0.0037 | 4.0 |
104 (50 fg) | 0.22 ± 0.0067 | 8.0 |
103 (5 fg) | 0.19 ± 0.0107 | 21.3 |
This calculation excludes the S282 codon, which was expected to have 10% variant mixture.