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The extraction method impacts the MALDI-TOF quality score. Ten NTM isolates were extracted in parallel with either the Vitek extraction method or the Bruker extraction method and then analyzed. (A) The Biotyper generated higher scores for samples extracted with the Bruker method. (B) The Saramis generated higher confidence values and was more likely to identify samples extracted with the Vitek method. (C) Vitek MS v3.0 was relatively insensitive to the extraction method.
