Table 1.
List of primers for quantitative PCR analysis of colostrum-regulated gene targets identified by microarray analysis
| Gene symbol | RefSeqAccession | Primer | Sequence (5′– > 3′) | Start | Stop |
|---|---|---|---|---|---|
| AREG | NM_214376 | Forward | CACAGCTCCGCGGGACCAAT | 172 | 191 |
| Reverse | CCGTTGACGTCCAGTCCAGCA | 281 | 261 | ||
| CDKN2B | NM_214124 | Forward | GCAAGTGGAGACCGTGCGTCA | 82 | 102 |
| Reverse | CACGTGCGCTGCCCATCATCA | 188 | 168 | ||
| CXCR4 | NM_213773 | Forward | AAGGATGGACGCGCCACACA | 23 | 42 |
| Reverse | GCATCCGAGAAGCGTGCCCC | 123 | 104 | ||
| F3 | NM_213785 | Forward | AAGTCGCATTGAGTGCACCAGCC | 818 | 840 |
| Reverse | ACACAGGCGGGACGAGGACG | 927 | 908 | ||
| HAS3 | NM_001001268 | Forward | GCCGGCTTCTTTGTGTGGCG | 436 | 455 |
| Reverse | TGCTGGCCCGCACCACATTC | 544 | 525 | ||
| HBEGF | NM_214299 | Forward | CGTCGGTGGTGCTGAAGCTCT | 14 | 34 |
| Reverse | ATTGCTGGTTCCATCCGCCAGC | 117 | 96 | ||
| IL-8 | NM_213867 | Forward | CAGCCCGTGTCAACATGACTTCCA | 70 | 93 |
| Reverse | GCACTGGCATCGAAGTTCTGCAC | 191 | 169 | ||
| NGF | XM_001929549 | Forward | CGAGAGGGAGAGGGTGGCTGG | 18 | 38 |
| Reverse | AACGCCCACCTGCTTGTTGC | 117 | 98 | ||
| PLAU | NM_213945 | Forward | GGTGAGAGTCACCGGCCTGC | 570 | 589 |
| Reverse | TAAAGCGGGGCCTCAGAGCCT | 669 | 649 | ||
| PTGS1 | XM_001926129 | Forward | GCACCGGTGAACCCCTGTTGT | 126 | 146 |
| Reverse | CAGAGTAGCCCGTGCGGGTG | 231 | 212 | ||
| SERPINE1 | NM_213910 | Forward | CCACCCCCGACGGCCATTAC | 847 | 866 |
| Reverse | TGGTGAGGGCGGAGAGAGGC | 960 | 941 | ||
| SMAD7 | NM_001244175 | Forward | GATGCTGTGCCTTCCTCCGCT | 676 | 696 |
| Reverse | GTGACCGATCCCCAGGCTCCA | 775 | 755 | ||
| TAGAP | NM_001243543 | Forward | CGGCACATTCCAGCGTTGCC | 1014 | 1033 |
| Reverse | ACGTCGGGGTCGGGATCGTT | 1125 | 1106 | ||
| β-actin | XM_003357928 | Forward | CTCTTCCAGCCCTCCTTCCT | 447 | 466 |
| Reverse | GCGTAGAGGTCCTTCCTGATGT | 518 | 497 | ||
| PPIA | NM_214353 | Forward | TGCAGACAAAGTTCCAAAGACAG | 75 | 97 |
| Reverse | GCCACCAGTGCCATTATGG | 225 | 207 |