Fig 5. Recall CD8⁺ T cell responses are not compromised in mice with oseltamivir-interrupted primary influenza infection.

(A) Naïve female BL/6 mice were administered either oseltamivir or PBS four hours prior to intranasal infection with 10⁴ pfu of HK and then once daily for eight days. Mice were secondarily challenged i.n. with 600 pfu of PR8 either (CD) 55 or (EF) 120 days after primary infection. (B) Mice were monitored daily for body weight loss. D^bNP₃₆₆ and D^bPA₂₂₄-specific CD8⁺ T cells in (CE) BAL and (DF) spleen were enumerated by intracellular staining of IFN-γ after five hours of stimulation with NP₃₆₆ or PA₂₂₄ peptide. Data represent the mean and standard deviation of a single experiment with 4–5 mice per group. Similar results were observed from two further recall experiments at day 55 after primary infection.