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. 2015 Mar 15;5(4):1319–1336.

Figure 3.

Figure 3

[6]-gingerol decreased FASN protein expression and de-novo fatty synthesis in HepG2 cells. Cells were treated with [6]-gingerol for 24 h and 48 h or 0.1% DMSO vehicle. A. Western blots of [6]-gingerol treatment for 24 I or 48 h II for FASN, ACC, ACLY antibodies and β-actin. BI. Confocal images of HepG2 cell cultures treated with vehicle or with 0.5 mM [6]-gingerol for 24 or 48 h. Cells were fixed and labelled with either anti-FASN or anti-β-actin antibodies and Alexa Fluor 488 Goat Anti-Rabbit IgG (H+L) antibody (green) and DAPI (blue). BII Quantitation of FASN fluorescence relative to β-actin. C. Long chain fatty acid contents of HepG2 cells after treatment with [6]-gingerol (0.25 or 0.5 mM) for 24 I or 48 h II expressed relative to that in cells treated with vehicle only. Each data point is the mean ± SD of 3 independent experiments. *P<0.05 comparing vehicle only.