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Netrin-1 regulates BVES expressions by PI3K/AKT pathway. (A) 100ng/ml rhnetrin-1 increased p-AKT level in a time-dependent manner, while total AKT level was not obviously increased. 50 µ mol/L LY294002 pretreatment decreased p-AKT level to a large extent (B), and restored the decreased expression of BVES by rhnetrin-1 to a great extent, as detected by western blot (B) and immunofluorescence staining (C).
