Figure 4.

BVES inhibits migration and invasion of HCC cells. Scratch wound healing assay (A) and Transwell chambers assays (B) were used to analyze cell migration and invasion, BVES knockdown promoted Huh7 cells migration and invasion, while overexpression of BVES in SK-Hep-1 cells suppressed them. (C) EMT markers E-cadherin and vimentin were detected after BVES knockdown and overexpression. (D) BVES up-regulated ZO-1 expression, and decreased RhoA activity as detected by RhoA-GTPase activation assay. In 3D culture assay, down-regulation of BVES in Huh7 cells increased the invasion capability (E), while overexprssion of BVES inhibited the invasion capability in SK-Hep-1 cells (F).