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. 2015 Mar 15;5(4):1447–1459.

Figure 3.

Figure 3

CTHRC1 is a direct target of miR-520d-5p. A. Predicted miR-520d-5p binding sequences in the 3’UTR of CTHRC1. The sequences of miR-520d-5p and wild-type (Wt) or mutant (Mut) putative target sites were compared in the 3’UTR of CTHRC1 mRNA. B. Expression of CTHRC1 in CRC tissues. (left) Real-time PCR analysis of CTHRC1 expression in 52 paired CRC tissues and the matched adjacent normal tissues. (right) Western blot analyses of CTHRC1 expression in eight paired CRC tissues. C. Inverse correlation between miR-520d-5p and CTHRC1 mRNA in 30 paired clinical tissue samples. D. The changes in CTHRC1 expression after transfection of miR-520d-5p mimics (left) and inhibitor (right) in CRC cell lines. E. Relative luciferase activity of the Wt-CTHRC1-3’UTR or Mut-CTHRC1-3’UTR or pmirGLO-vector after transfection with miR-520d-5p mimics and negative control in SW620 cells (top) or 20 and 50 pmol miR-520d-5p mimics in LoVo and HEK293 cells (bottom). Values, mean ± SD of three independent assays. *P < 0.05; **P < 0.01; ***P < 0.001.