Table 1.
Primers used for RT-PCR and RT-qPCR analyses
| Gene | Forward primer (5’-3’) | Ta (°C)a | Amplicon size (bp) |
|---|---|---|---|
|
| |||
| Reverse primer (5’-3’) | |||
| RHOT1 | F: GGGAGGAACCTCTTCTGGA | 60 | 105 |
| R: ATGAAGAAAGACGTGCGGAT | |||
| GAPDH | F: TGCACCACCAACTGCTTAGC | 60 | 87 |
| R: GGCATGGACTGTGGTCATGAG | |||
| SMAD4 | F: GCTGCTGGAATTGGTGTTGATG | 60 | 108 |
| R: AGGTGTTTCTTTGATGCTCTGTCT | |||
| P53 | F: TCAACAAGATGTTTTGCCAACTG | 60 | 118 |
| R: ATGTGCTGTGACTGCTTGTAGATG | |||
| P16 | F: CCCTCGTGCTGATGCTACTG | 60 | 72 |
| R: CATCATGACCTGGTCTTCTAGGAA | |||
| Kras | F: ACAGAAGTGGAGGATGCTTT | 60 | 100 |
| R: TTTCACACAGCCAGGAGTCTT |
a
annealing temperature for PCR amplification.