Figure 4.

ALDH1A1 glioma cells are capable of colony formation and proliferation. A. Colony formation of FACS-sorted ALDH1A1⁺ and ALDH1A1^- cells yielded from U251 and primary glioblastoma cells (left). Quantitative analysis shows significant difference between colony formation by ALDH1A1⁺ and that by ALDH1A1^- cells (right). B. Quantitative analysis by cell counting kit-8 (CCK8) assay shows significant difference between proliferation capability by ALDH1^high and that by ALDH1^low cells isolated from U251 and primary glioma cells. C. Kaplan Meier survival curve of overall survival (OS) indicates the association between FACS-sorted ALDH1A1⁺ and ALDH1A1^- cells of U251 cells in SCID. Animals xenografted with ALDH1A1⁺ cells had a mean OS of 64 days as compared to 89 days for those with ALDH1A1^- ones. (P = 0.001, log-rank test). Each value is the mean ± SEM. The Student’s t-test was used for between ALDH1A1^high and ALDH1A1^low analysis. *indicates significant difference (P < 0.05), **P < 0.01, ***P < 0.001.