Figure 2.

Establishment of stable cell lines of ectopic expression and knockdown of S100A14. A. The protein levels of S100A14 were detected in SiHa, HeLa, C33A and CaSKi cells. β-actin was used as a loading control. B, C. SiHa and C33A cells were infected with pLVX-Con and pLVX-S100A14 lentivirus, stable cells were established by Geneticin (G418) selection for about 2 weeks. Cells were harvested. S100A14 expression was detected by qRT-PCR (Left panel: [mean (n = 2) ± SD; 2-sided t test; *P < 0.05; **P < 0.01, normalized to β-actin]) and Western Blot (Right panel). And cells infected by pLVX-S100A14 lentivirus were named as SiHa-pLVX-S100A14, C33A-pLVX-S100A14 and the corresponding control cells were named as SiHa-pLVX-Control and C33A-pLVX-Control respectively. D. CaSKi cells were infected with shGFP, sh-S100A14-1#, and sh-S100A14-2# lentivirus, stable cells were established by puromycin selection for about 4 days. Cells were harvested. S100A14 expression was detected by qRT-PCR (Left panel: [mean (n = 2) ± SD; 2-sided t test; *P < 0.05, normalized to β-actin]) and Western Blot (Right panel). And cells infected by lentivirus containing two shRNA sequences targeting human S100A14 were named as CaSKi-shS100A14-1# and CaSKi-shS100A14-2# respectively, and the corresponding control cells were named as CaSKi-shGFP.