Figure 2.

KDM4 and H3 mark expression in breast cancer. A. mRNA expression levels of KDM4 demethylases in a panel of breast cancer cell lines were determined by qRT-PCR. mRNA expression levels in the MCF10A cells, an immortalized but nontumorigenic breast epithelial cell line, were arbitrarily set as 1. Relative expression levels in breast cancer cell lines are shown as fold changes compared with that of MCF10A cells. *The HCC1954 line belongs to the basal-like subtype even though it contains HER2 amplification. B. KDM4A, B, C, D and ER protein levels were analyzed by western blot in eight breast cancer cell lines and MCF10A line. Total H3 was used as the loading control. C. Global H3K4, K9, and K36 methylation levels in seven breast cancer cell lines and MCF10A line. Histone extracts were prepared from cells using the EpiQuik Total Histone Extraction Kit. Global histone methylation levels were detected by western blot assays with specific histone methylation antibodies. Total H3 level was used as the loading control.