Figure 3.

Hyperplasia of TH-MYCN mice predominantly consisted of undifferentiated progenitor cells. Sections of sympathetic ganglia from wild-type and TH-MYCN mice were immunostained for two neuron markers, Tuj1 and TH. The majority of cells in the wild-type sympathetic ganglia and the non-hyperplastic region of TH-MYCN sympathetic ganglia, displayed the morphology of mature neurons and expressed Tuj1 and TH. However, the hyperplastic lesion of TH-MYCN sympathetic ganglia stained negatively for Tuj1 or TH and displayed a small blue round cell morphology. TH-MYCN, tyrosine hydroxylase v-myc avian myelocytomatosis viral oncogene neuroblastoma derived homolog; Phox2B, paired-like homeobox 2b; H&E, hematoxylin and eosin.