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MSC-driven osteogenesis in monolayer cultures (magnification, ×400). (A) Positive von Kossa staining in a monolayer culture of human bone marrow-derived MSCs exposed to osteogenic medium for three weeks. (B) Control monolayer cultures of MSCs grown with basic medium (no osteogenic supplement) for three weeks showed a negative reaction to von Kossa staining. MSC, mesenchymal stem cell.
