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Serial dilution of p-nitrophenol standard (200 µg/ml) for concentrations of 0–200 µg/ml.
| Serial dilution standard p-nitrophenol | Volume of 200 µg/ml solution (µl) | Volume of distilled water (µl) | Concentration (µg/ml) |
|---|---|---|---|
| A | 0.000 | 200.0 | 0.000 |
| B | 3.125 | 196.9 | 3.125 |
| C | 6.250 | 193.8 | 6.250 |
| D | 12.500 | 187.5 | 12.500 |
| E | 25.000 | 175.0 | 25.000 |
| F | 50.000 | 150.0 | 50.000 |
| G | 100.000 | 100.0 | 100.000 |
| H | 200.000 | 0.0 | 200.000 |
